KMID : 0371420211000030144
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Annals of Surgical Treatment and Research 2021 Volume.100 No. 3 p.144 ~ p.153
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Diagnostic model for pancreatic cancer using a multi-biomarker panel
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Choi Yoo-Jin
Yoon Woong-Chang Lee A-Reum Byun Yoon-Hyeong Kang Jae-Seung Kim Hong-Beom Kwon Woo-Il Suh Young-Ah Kim Yong-Kang Lee Seung-Yeoun Namkung Jung-Hyun Han Sang-Jo Choi Yong-Hwan Heo Jin-Seok Park Joon-Oh Park Joo-Kyung Kim Song-Cheol Kang Chang-Moo Lee Woo-Jin Park Tae-Sung Jang Jin-Young
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Abstract
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Purpose: Diagnostic biomarkers of pancreatic ductal adenocarcinoma (PDAC) have been used for early detection to reduce its dismal survival rate. However, clinically feasible biomarkers are still rare. Therefore, in this study, we developed an automated multi-marker enzyme-linked immunosorbent assay (ELISA) kit using 3 biomarkers (leucine-rich alpha-2-glycoprotein [LRG1], transthyretin [TTR], and CA 19-9) that were previously discovered and proposed a diagnostic model for PDAC based on this kit for clinical usage.
Methods: Individual LRG1, TTR, and CA 19-9 panels were combined into a single automated ELISA panel and tested on 728 plasma samples, including PDAC (n = 381) and normal samples (n = 347). The consistency between individual panels of 3 biomarkers and the automated multi-panel ELISA kit were accessed by correlation. The diagnostic model was developed using logistic regression according to the automated ELISA kit to predict the risk of pancreatic cancer (high-, intermediate-, and low-risk groups).
Results: The Pearson correlation coefficient of predicted values between the triple-marker automated ELISA panel and the former individual ELISA was 0.865. The proposed model provided reliable prediction results with a positive predictive value of 92.05%, negative predictive value of 90.69%, specificity of 90.69%, and sensitivity of 92.05%, which all simultaneously exceed 90% cutoff value.
Conclusion: This diagnostic model based on the triple ELISA kit showed better diagnostic performance than previous markers for PDAC. In the future, it needs external validation to be used in the clinic.
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KEYWORD
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Biomarkers, Enzyme-linked immunosorbent assay, Pancreatic intraductal neoplasms
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